06A.02A · Réplication de l'ADN

DNA replication

SMB1 De la molécule à la cellule De l'ADN à la protéine · 51 pages

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DNA replication

DNA Replication

1DNA Replication

2DNA-to-DNA Loop

3Lecture Agenda

Semi-conservative Replication

5Three Replication Models 6Meselson-Stahl Experiment 7Meselson-Stahl Results 8Strand Complementarity 9S Phase Timing

10Lecture Agenda

Replication Eyes & Forks

11Replication Eyes & Forks 12Replication Domains & Timing

Quiz: Genome Replication Time

13Quiz: Genome Replication Time

14→ Replicative Mix

Replicative Mix

15Four Required Ingredients

16→ DNA Synthesis

17Nucleotide & Phosphate Groups 185′→3′ Directionality 195′→3′ Chain Growth 20Polymerase Action Steps

Replicative Mix

21DNA Pol III Complex

22Helicase Unwinds DNA

23→ Replication Initiation

Replication Initiation

24Helicase, SSB & Primase 25Origin Loading Sequence 26Origin Genome Regions

27→ Replication Elongation

Quiz: Polymerase Speed

28Quiz: Polymerase Speed

Replication Elongation

29Leading & Lagging Strands 30Okazaki Fragment Processing

31Lecture Break

Replication Elongation

32Eukaryotic Fork Machinery

33→ Replication Fidelity

Replication Fidelity

34Proofreading (3′→5′ Exonuclease)35Mismatch Repair

36→ Topological Problem

Topological Problem

37Torsional Stress & Topo I 38Topo I Catalytic Mechanism 39Topo II: Double-Strand Gate

40→ Telomeres

41End-Replication Problem 42Telomerase Extends Ends 43Short Telomere Syndromes

44→ Applications

45Nucleoside Analogues (AZT)46Topoisomerase Inhibitors 47PCR: First Cycle 48PCR: Exponential Amplification 49Quantitative PCR (qPCR)50RT-PCR: COVID-19 Detection

06A.02APage 1DNA Replication

Réplication de l'ADN page 1

06A.02APage 2Central DogmaDNA-to-DNA Loop

Réplication de l'ADN page 2

06A.02APage 3Lecture Agenda

Réplication de l'ADN page 3

06A.02APage 4Lecture Agenda

Réplication de l'ADN page 4

06A.02APage 5Semi-conservative ReplicationThree Replication Models

Réplication de l'ADN page 5

06A.02APage 6Semi-conservative ReplicationMeselson-Stahl Experiment

Réplication de l'ADN page 6

06A.02APage 7Semi-conservative ReplicationMeselson-Stahl Results

Réplication de l'ADN page 7

06A.02APage 8Semi-conservative ReplicationStrand Complementarity

Réplication de l'ADN page 8

06A.02APage 9Semi-conservative ReplicationS Phase Timing

Réplication de l'ADN page 9

06A.02APage 10Lecture Agenda

Réplication de l'ADN page 10

06A.02APage 11Replication Eyes & Forks

Réplication de l'ADN page 11

06A.02APage 12Replication Eyes & ForksReplication Domains & Timing

Réplication de l'ADN page 12

06A.02APage 13Quiz: Genome Replication Time

Réplication de l'ADN page 13

06A.02APage 14Lecture Agenda→ Replicative Mix

Réplication de l'ADN page 14

06A.02APage 15Replicative MixFour Required Ingredients

Réplication de l'ADN page 15

06A.02APage 16Lecture Agenda→ DNA Synthesis

Réplication de l'ADN page 16

06A.02APage 17DNA SynthesisNucleotide & Phosphate Groups

Réplication de l'ADN page 17

06A.02APage 18DNA Synthesis5′→3′ Directionality

Réplication de l'ADN page 18

06A.02APage 19DNA Synthesis5′→3′ Chain Growth

Réplication de l'ADN page 19

06A.02APage 20DNA SynthesisPolymerase Action Steps

Réplication de l'ADN page 20

06A.02APage 21Replicative MixDNA Pol III Complex

Réplication de l'ADN page 21

06A.02APage 22DNA SynthesisHelicase Unwinds DNA

Réplication de l'ADN page 22

06A.02APage 23Lecture Agenda→ Replication Initiation

Réplication de l'ADN page 23

06A.02APage 24Replication InitiationHelicase, SSB & Primase

Réplication de l'ADN page 24

06A.02APage 25Replication InitiationOrigin Loading Sequence

Réplication de l'ADN page 25

06A.02APage 26Replication InitiationOrigin Genome Regions

Réplication de l'ADN page 26

06A.02APage 27Lecture Agenda→ Replication Elongation

Réplication de l'ADN page 27

06A.02APage 28Quiz: Polymerase Speed

Réplication de l'ADN page 28

06A.02APage 29Replication ElongationLeading & Lagging Strands

Réplication de l'ADN page 29

06A.02APage 30Replication ElongationOkazaki Fragment Processing

Réplication de l'ADN page 30

06A.02APage 31Lecture Break

Réplication de l'ADN page 31

06A.02APage 32Replication ElongationEukaryotic Fork Machinery

Réplication de l'ADN page 32

06A.02APage 33Lecture Agenda→ Replication Fidelity

Réplication de l'ADN page 33

06A.02APage 34Replication FidelityProofreading (3′→5′ Exonuclease)

Réplication de l'ADN page 34

06A.02APage 35Replication FidelityMismatch Repair

Réplication de l'ADN page 35

06A.02APage 36Lecture Agenda→ Topological Problem

Réplication de l'ADN page 36

06A.02APage 37Topological ProblemTorsional Stress & Topo I

Réplication de l'ADN page 37

06A.02APage 38Topological ProblemTopo I Catalytic Mechanism

Réplication de l'ADN page 38

06A.02APage 39Topological ProblemTopo II: Double-Strand Gate

Réplication de l'ADN page 39

06A.02APage 40Lecture Agenda→ Telomeres

Réplication de l'ADN page 40

06A.02APage 41TelomeresEnd-Replication Problem

Réplication de l'ADN page 41

06A.02APage 42TelomeresTelomerase Extends Ends

Réplication de l'ADN page 42

06A.02APage 43TelomeresShort Telomere Syndromes

Réplication de l'ADN page 43

06A.02APage 44Lecture Agenda→ Applications

Réplication de l'ADN page 44

06A.02APage 45ApplicationsNucleoside Analogues (AZT)

Réplication de l'ADN page 45

06A.02APage 46ApplicationsTopoisomerase Inhibitors

Réplication de l'ADN page 46

06A.02APage 47ApplicationsPCR: First Cycle

Réplication de l'ADN page 47

06A.02APage 48ApplicationsPCR: Exponential Amplification

Réplication de l'ADN page 48

06A.02APage 49ApplicationsQuantitative PCR (qPCR)

Réplication de l'ADN page 49

06A.02APage 50ApplicationsRT-PCR: COVID-19 Detection

Réplication de l'ADN page 50

06A.02APage 51Blank Slide

Réplication de l'ADN page 51