09A.04 · Enzymes II — régulation et coenzymes

Enzymes II — regulation and coenzymes

Biochimie et métabolisme · 61 pages

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Enzymes 2: Title Slide

1Enzymes 2: Title Slide

Course Overview

2Course Overview

3Lecture Agenda

09A.04Page 1Enzymes 2: Title Slide

Enzymes II — régulation et coenzymes page 1

09A.04Page 2Course Overview

Enzymes II — régulation et coenzymes page 2

09A.04Page 3Lecture Agenda

Enzymes II — régulation et coenzymes page 3

09A.04Page 4Metabolic RegulationSupply-Demand Concept

Enzymes II — régulation et coenzymes page 4

09A.04Page 5Metabolic RegulationGlucose Pathway Overview

Enzymes II — régulation et coenzymes page 5

09A.04Page 6Metabolic RegulationGlucose Pathway Overview

Enzymes II — régulation et coenzymes page 6

09A.04Page 7Metabolic RegulationRate-Limiting Reaction

Enzymes II — régulation et coenzymes page 7

09A.04Page 8Metabolic RegulationRate-Limiting Reaction

Enzymes II — régulation et coenzymes page 8

09A.04Page 9Metabolic RegulationRate-Limiting Reaction

Enzymes II — régulation et coenzymes page 9

09A.04Page 10Metabolic RegulationRate-Limiting Reaction Speed

Enzymes II — régulation et coenzymes page 10

09A.04Page 11Metabolic RegulationSubstrate Availability

Enzymes II — régulation et coenzymes page 11

09A.04Page 12Metabolic RegulationEnzyme Activity Control

Enzymes II — régulation et coenzymes page 12

09A.04Page 13Metabolic RegulationEnzyme Activity ControlPhosphorylation/Dephosphorylation

Enzymes II — régulation et coenzymes page 13

09A.04Page 14Metabolic RegulationEnzyme Activity ControlSignal Amplification Cascade

Enzymes II — régulation et coenzymes page 14

09A.04Page 15Metabolic RegulationEnzyme Activity ControlSignal Amplification Cascade

Enzymes II — régulation et coenzymes page 15

09A.04Page 16Metabolic RegulationEnzyme Activity ControlAllosteric Control

Enzymes II — régulation et coenzymes page 16

09A.04Page 17Metabolic RegulationEnzyme Activity ControlAllosteric Control

Enzymes II — régulation et coenzymes page 17

09A.04Page 18Metabolic RegulationEnzyme Activity ControlAllosteric Control

Enzymes II — régulation et coenzymes page 18

09A.04Page 19Metabolic RegulationEnzyme Activity ControlAllosteric Control

Enzymes II — régulation et coenzymes page 19

09A.04Page 20Metabolic RegulationEnzyme Activity ControlAllosteric vs Non-competitive

Enzymes II — régulation et coenzymes page 20

09A.04Page 21Metabolic RegulationEnzyme Activity ControlMechanisms Summary

Enzymes II — régulation et coenzymes page 21

09A.04Page 22Metabolic RegulationEnzyme Concentration Control

Enzymes II — régulation et coenzymes page 22

09A.04Page 23Metabolic RegulationEnzyme Concentration Control

Enzymes II — régulation et coenzymes page 23

09A.04Page 24Metabolic RegulationRegulation Summary

Enzymes II — régulation et coenzymes page 24

09A.04Page 25Metabolic RegulationControl TimescalesAllosteric (≤1 s)

Enzymes II — régulation et coenzymes page 25

09A.04Page 26Metabolic RegulationControl TimescalesCovalent Modification (s–min)

Enzymes II — régulation et coenzymes page 26

09A.04Page 27Metabolic RegulationControl TimescalesTranscriptional (hours–months)

Enzymes II — régulation et coenzymes page 27

09A.04Page 28Metabolic RegulationControl TimescalesTranscriptional (hours–months)

Enzymes II — régulation et coenzymes page 28

09A.04Page 29Metabolic RegulationControl Timescales Summary

Enzymes II — régulation et coenzymes page 29

09A.04Page 30CoenzymesDefinition and Properties

Enzymes II — régulation et coenzymes page 30

09A.04Page 31CoenzymesCoenzyme Transfer Function

Enzymes II — régulation et coenzymes page 31

09A.04Page 32CoenzymesMechanisms and Functions

Enzymes II — régulation et coenzymes page 32

09A.04Page 33CoenzymesCosubstrate Mechanism

Enzymes II — régulation et coenzymes page 33

09A.04Page 34CoenzymesProsthetic Group Mechanism

Enzymes II — régulation et coenzymes page 34

09A.04Page 35CoenzymesOxidoreduction CoenzymesReducing Equivalents

Enzymes II — régulation et coenzymes page 35

09A.04Page 36CoenzymesOxidoreduction CoenzymesRedox Coenzyme Types

Enzymes II — régulation et coenzymes page 36

09A.04Page 37CoenzymesOxidoreduction CoenzymesOxidoreductase Cofactors

Enzymes II — régulation et coenzymes page 37

09A.04Page 38CoenzymesOxidoreduction CoenzymesDehydrogenase/NAD Example

Enzymes II — régulation et coenzymes page 38

09A.04Page 39CoenzymesNAD and NADPNiacin (Vitamin B3)

Enzymes II — régulation et coenzymes page 39

09A.04Page 40CoenzymesNAD and NADPNiacin Deficiency (Pellagra)

Enzymes II — régulation et coenzymes page 40

09A.04Page 41CoenzymesNAD and NADPNAD Structure

Enzymes II — régulation et coenzymes page 41

09A.04Page 42CoenzymesNAD and NADPNAD Redox Couple

Enzymes II — régulation et coenzymes page 42

09A.04Page 43CoenzymesNAD and NADPNADH Absorption Spectrum

Enzymes II — régulation et coenzymes page 43

09A.04Page 44CoenzymesNAD and NADPNADH Assay at 340 nm

Enzymes II — régulation et coenzymes page 44

09A.04Page 45CoenzymesNAD and NADPEnzyme Kinetics: Vmax

Enzymes II — régulation et coenzymes page 45

09A.04Page 46CoenzymesNAD and NADPNADH Assay at 340 nm

Enzymes II — régulation et coenzymes page 46

09A.04Page 47CoenzymesNAD and NADPNAD Redox Mechanism

Enzymes II — régulation et coenzymes page 47

09A.04Page 48CoenzymesNAD and NADPNAD in Catabolism

Enzymes II — régulation et coenzymes page 48

09A.04Page 49CoenzymesNAD and NADPNAD in Catabolism

Enzymes II — régulation et coenzymes page 49

09A.04Page 50CoenzymesNADP in Anabolism

Enzymes II — régulation et coenzymes page 50

09A.04Page 51CoenzymesFADFAD Properties

Enzymes II — régulation et coenzymes page 51

09A.04Page 52CoenzymesFADRiboflavin Deficiency

Enzymes II — régulation et coenzymes page 52

09A.04Page 53CoenzymesFADFAD Structure

Enzymes II — régulation et coenzymes page 53

09A.04Page 54CoenzymesFADFAD in Mitochondria

Enzymes II — régulation et coenzymes page 54

09A.04Page 55CoenzymesNAD and FAD in Catabolism

Enzymes II — régulation et coenzymes page 55

09A.04Page 56CoenzymesNADP in Anabolism

Enzymes II — régulation et coenzymes page 56

09A.04Page 57CoenzymesGroup Transfer Coenzymes

Enzymes II — régulation et coenzymes page 57

09A.04Page 58CoenzymesCoenzyme ACoA Structure

Enzymes II — régulation et coenzymes page 58

09A.04Page 59CoenzymesCoenzyme ACoA Function

Enzymes II — régulation et coenzymes page 59

09A.04Page 60CoenzymesCoenzyme Classification Summary

Enzymes II — régulation et coenzymes page 60

09A.04Page 61CoenzymesIntegrated Metabolic Map

Enzymes II — régulation et coenzymes page 61