09A.05 · Glycolyse

Glycolysis

Biochimie et métabolisme · 55 pages

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Glycolysis Introduction

1Glycolysis Introduction

2Course Outline

Lecture Roadmap

3Lecture Roadmap

Glycolysis in Metabolism

4Glycolysis in Metabolism

Glucose Pathways

5Glucose Pathways

Glycolysis Definition

6Glycolysis Definition

Pathway Overview

7Pathway Overview

8Enzyme Classes

Reactions on Pathway

9Reactions on Pathway

Investment Phase

10Investment Phase

14Pyruvate Fates 15Aerobic vs Anaerobic

ATP Yield Comparison

16ATP Yield Comparison

Biosynthetic Precursors

18Biosynthetic Precursors

Tissue Glucose Use

19Tissue Glucose Use

Glucose Storage

20Glucose Storage

Glucose Sources

21Glucose Sources

Two-Phase Review

22Two-Phase Review

Regulatory Enzymes

23Step 1: Hexokinase 24Hexokinase vs Glucokinase 25Km Comparison 26Glucose-6P Branch Point 27Hexokinase Regulation 28Step 3: PFK Control 29PFK Allosteric Regulation

Step-by-Step Reactions

30Investment Phase Steps 31Payoff Phase Steps 32Step 6: G3P Oxidation 33Substrate Phosphorylation

34ΔG°' of All Steps 35Irreversible Steps 36Mass Action 37Cellular ΔG Calculation 38Irreversibility Confirmed

39NADH Production 40Aerobic vs Anaerobic Routes 43NAD+ Regeneration Requirement 44Anaerobic: Lactate Route 45Aerobic: Mitochondrial Route 46Anaerobic: Lactate DH 47NADH Shuttles Overview 48Glycerophosphate Shuttle 51Malate/Aspartate Shuttle

Metabolic Adaptation

54Metabolic Adaptation

Pyruvate Fates Summary

55Pyruvate Fates Summary

09A.05Page 1Glycolysis Introduction

Glycolyse page 1

09A.05Page 2Course Outline

Glycolyse page 2

09A.05Page 3Lecture Roadmap

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09A.05Page 4Glycolysis in Metabolism

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09A.05Page 5Glucose Pathways

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09A.05Page 6Glycolysis Definition

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09A.05Page 7Pathway Overview

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09A.05Page 8Enzyme Classes

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09A.05Page 9Reactions on Pathway

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09A.05Page 10Investment Phase

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09A.05Page 11Payoff Phase

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09A.05Page 12Full Pathway

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09A.05Page 13Net Balance

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09A.05Page 14Pyruvate Fates

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09A.05Page 15Pyruvate FatesAerobic vs Anaerobic

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09A.05Page 16ATP Yield Comparison

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09A.05Page 17ATP Balance

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09A.05Page 18Biosynthetic Precursors

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09A.05Page 19Tissue Glucose Use

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09A.05Page 20Glucose Storage

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09A.05Page 21Glucose Sources

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09A.05Page 22Two-Phase Review

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09A.05Page 23Regulatory EnzymesStep 1: Hexokinase

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09A.05Page 24Regulatory EnzymesHexokinase vs Glucokinase

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09A.05Page 25Regulatory EnzymesKm Comparison

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09A.05Page 26Regulatory EnzymesGlucose-6P Branch Point

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09A.05Page 27Regulatory EnzymesHexokinase Regulation

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09A.05Page 28Regulatory EnzymesStep 3: PFK Control

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09A.05Page 29Regulatory EnzymesPFK Allosteric Regulation

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09A.05Page 30Step-by-Step ReactionsInvestment Phase Steps

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09A.05Page 31Step-by-Step ReactionsPayoff Phase Steps

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09A.05Page 32Step-by-Step ReactionsStep 6: G3P Oxidation

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09A.05Page 33Step-by-Step ReactionsSubstrate Phosphorylation

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09A.05Page 34ThermodynamicsΔG°' of All Steps

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09A.05Page 35ThermodynamicsIrreversible Steps

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09A.05Page 36ThermodynamicsMass Action

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09A.05Page 37ThermodynamicsCellular ΔG Calculation

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09A.05Page 38ThermodynamicsIrreversibility Confirmed

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09A.05Page 39NADH FatesNADH Production

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09A.05Page 40NADH FatesAerobic vs Anaerobic Routes

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09A.05Page 41NADH FatesAerobic vs Anaerobic Routes

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09A.05Page 42NADH FatesAerobic vs Anaerobic Routes

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09A.05Page 43NADH FatesNAD+ Regeneration Requirement

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09A.05Page 44NADH FatesAnaerobic: Lactate Route

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09A.05Page 45NADH FatesAerobic: Mitochondrial Route

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09A.05Page 46NADH FatesAnaerobic: Lactate DH

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09A.05Page 47NADH FatesNADH Shuttles Overview

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09A.05Page 48NADH FatesGlycerophosphate Shuttle

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09A.05Page 49NADH FatesGlycerophosphate Shuttle

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09A.05Page 50NADH FatesGlycerophosphate Shuttle

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09A.05Page 51NADH FatesMalate/Aspartate Shuttle

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09A.05Page 52NADH FatesMalate/Aspartate Shuttle

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09A.05Page 53NADH FatesMalate/Aspartate Shuttle

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09A.05Page 54Metabolic Adaptation

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09A.05Page 55Pyruvate Fates Summary

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